For the diagnostic fields of thrombophilia, metabolism and immunogenetics we offer Realtime LoopTag (LT) PCR assays for the detection of disease-related polymorphisms in the human genome. That quick and certain method assures the reliable genotyping of patient samples. The tests of the product line Realtime LT are compatible with our nucleic acid extraction kit Attosorb 96.

After the isolation of DNA from patient’s blood the target sequence is amplified within a single reaction mixture. The detection of amplification products is performed by the hybridization of specific LoopTag probes1). The definite identification of the sample’s genotype is made by a melting curve analysis after the amplification.