For the detection of disease-related polymorphisms in the human genome in the diagnostic fields of thrombophilia, metabolism and immunogenetics we also offer real-time PCR assays based on TaqManTM technology. That quick procedure assures reliable genotyping of patients samples on various devices. The tests of the product line Realtime TM are compatible with the nucleic acid extraction kit Attosorb 96.

After the isolation of DNA from patient's blood the target sequence is amplified within a single reaction mixture. The specific TM probe is degraded through the 5`-nuclease activity of the polymerase in each amplification cycle. The released fluorescent dyes can be detected in the corresponding channel of a real-time cycler. The evaluation is performed either by the direct analysis of amplification curves or easily interpretable scatter plot.